ChIP-seq

ChIP-seq (Chromatin Immunoprecipitation followed by sequencing) is a molecular biology technique used to analyze protein-DNA interactions across the genome. It combines chromatin immunoprecipitation with high-throughput DNA sequencing to identify binding sites of transcription factors, histone modifications, or other chromatin-associated proteins. This method provides genome-wide maps of protein-DNA interactions with high resolution and specificity.

Also known as: ChIP-Seq, ChIPseq, Chromatin Immunoprecipitation Sequencing, ChIP sequencing, ChIP Seq

Why learn ChIP-seq?

Developers should learn ChIP-seq when working in bioinformatics, computational biology, or genomics research, particularly for analyzing gene regulation, epigenetic modifications, or chromatin structure. It's essential for identifying transcription factor binding sites, studying histone modifications in diseases like cancer, or investigating developmental biology processes. Use cases include differential binding analysis, peak calling, and integrating with other omics data like RNA-seq.

Learning Resources

• docs: ENCODE ChIP-seq Guidelines
• tutorial: Introduction to ChIP-seq Analysis
• tutorial: ChIP-seq Analysis with Galaxy
• video: ChIP-seq: Overview and Data Analysis
• book: Bioinformatics Data Skills: ChIP-seq Chapter