Single Cell ATAC Sequencing

Single Cell ATAC Sequencing (scATAC-seq) is a high-throughput genomic technique used to profile chromatin accessibility at the single-cell level, enabling the identification of open chromatin regions that regulate gene expression. It combines Assay for Transposase-Accessible Chromatin (ATAC-seq) with single-cell sequencing technologies to map accessible DNA regions across individual cells, providing insights into cellular heterogeneity and regulatory dynamics. This tool is essential for studying epigenetics, cell differentiation, and disease mechanisms in complex tissues.

Also known as: scATAC-seq, Single-Cell ATAC-Seq, scATAC, ATAC-seq single cell, Single Cell Assay for Transposase-Accessible Chromatin

Why learn Single Cell ATAC Sequencing?

Developers should learn scATAC-seq when working in bioinformatics, computational biology, or genomics to analyze epigenetic data and understand gene regulation in diverse cell populations. It is particularly useful for applications in cancer research, developmental biology, and immunology, where identifying cell-type-specific regulatory elements is critical. Proficiency in scATAC-seq enables the development of pipelines for data preprocessing, integration with other omics data (e.g., scRNA-seq), and downstream analysis using tools like ArchR or Signac.

Learning Resources

• docs: scATAC-seq Analysis with Signac
• docs: ArchR: Analysis of Single-Cell Chromatin Accessibility
• tutorial: Introduction to Single-Cell ATAC-Seq
• course: Coursera Course: Single Cell ATAC-Seq Data Analysis
• video: YouTube: scATAC-seq Workflow Tutorial